Other
Part:BBa_K187423:Design
Designed by: Julia Pon Group: iGEM09_Alberta (2009-10-21)
Anchor forward strand A for Biobytes assembly method
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
See http://2009.igem.org/Team:Alberta/DNAanchor for binding capacity assays with the Biobytes anchor. We tested three alternative designs, which are documented on the wiki.
The anchoring system we decided on, and submitted here, is small in size and the presence of the ssDNA spacer region gives this anchor a high binding capacity of about 200 pmol mg-1 beads. The presence of the dsDNA region allows Bytes to be ligated to the anchor and the incorporation of a deoxyuracil containing I-SceI site allows the construct to be released via the two methods described already(USER and I-SceI digestion).
Source
Oligonucleotide synthesis